Targeted Editing of SlMAPK6 Using CRISPR/Cas9 Technology to Promote the Development of Axillary Buds in Tomato Plants

The mitogen-activated protein kinase (MAPK) cascade signaling system has been relatively conserved throughout the evolution of eukaryotes and is involved in regulation growth development metabolism. In this study, dwarf tomato plants were used as research material. First, tissue-specific expression SlMAPK6 was measured wild-type by quantitative RT-PCR. results showed that highly expressed tissues stems, leaves flowers but at low levels roots, sepals fruits. Second, SlMAPK6-knockout lines CRISPR-3 CRISPR-7 obtained CRISPR-Cas9 technology Agrobacterium-mediated transformation. Compared with wild-type, mutant significant phenotypic characteristics, such increased numbers axillary buds true leaves, thickened longer leaflets. addition, to explore molecular mechanism which MAPK regulates bud growth, we also positively strigolactone synthesis genes SlCCD7 SlCCD8 gibberellin (GA) GA20ox3 GA3ox1 negatively development-related Ls, BL BRC1b/TCP8 GA inhibitory gene GAI. Therefore, appears regulate induce buds.